{"$schema":"https://protein-truth-desk.manus.space/api/public/schemas/claims.schema.json","standard":"protein-truth-desk-verifiable-claims@1.0","generated_at":"2026-06-11T13:29:57.084Z","license":"https://creativecommons.org/licenses/by/4.0/","attribution":"Protein Truth Desk global verifiable-claims registry (CC BY 4.0)","count":200,"claims":[{"id":"ptd-270006-300043","value":"human growth hormone (hGH) in Escherichia coli BL21(DE3)","label":"Source organism claim","claim_type":"organism","extracted_value":"Escherichia coli BL21(DE3)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Escherichia coli BL21(DE3)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:14.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300043","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300039","value":"The resulting chum salmon head peptone (CSHP) exhibited favorable characteristics, including a low average molecular mass (557 Da)","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"average molecular mass (557 Da)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'average molecular mass (557 Da)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:12.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300039","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300042","value":"human growth hormone (hGH) in Escherichia coli BL21(DE3)","label":"Protein name claim","claim_type":"protein_name","extracted_value":"human growth hormone (hGH)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'human growth hormone (hGH)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:12.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300042","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300041","value":"CSHP was evaluated as a nitrogen source for recombinant protein production and supported higher expression of human superoxide dismutase (hSOD)","label":"Protein name claim","claim_type":"protein_name","extracted_value":"human superoxide dismutase (hSOD)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'human superoxide dismutase (hSOD)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:12.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300041","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300038","value":"Protamex achieved the highest hydrolysis and recovery rates.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Protamex","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Protamex' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:13.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300038","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300037","value":"chum salmon (Oncorhynchus keta)","label":"Source organism claim","claim_type":"organism","extracted_value":"Oncorhynchus keta","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Oncorhynchus keta' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:14.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300037","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270006-300040","value":"a high amino nitrogen content (4.9%)","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"amino nitrogen content (4.9%)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'amino nitrogen content (4.9%)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:52:12.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#claim-300040","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270006#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:52:06.000Z"},{"id":"ptd-270005-300036","value":"Complete residual media achieved high production (3.67, 2.46 and 30.83 g l(-1) of biomass, HA and LA respectively) and a high molecular weight of HA (approximately 2500 kDa).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"high molecular weight of HA (approximately 2500 kDa)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'high molecular weight of HA (approximately 2500 kDa)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:57.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270005#claim-300036","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270005#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:51.000Z"},{"id":"ptd-270005-300035","value":"Mussel processing wastewater (MPW) was used as a sugar source and tuna peptone (TP) from viscera residue as a protein substrate for the production of hyaluronic acid (HA), biomass and lactic acid (LA) by Streptococcus zooepidemicus in batch fermentation.","label":"Source organism claim","claim_type":"organism","extracted_value":"Streptococcus zooepidemicus","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Streptococcus zooepidemicus' not found in PubChem; [adapter] Manual review recommended [Audit note: The model's output should include both the 'Verdict' and 'Rationale' fields as presented in the optimized prompt. For example, it should output: {\"Verdict\": \"Insufficient Evidence\", \"Rationale\": \"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Streptococcus zooepidemicus' not found in PubChem; [adapter] Manual review recommended\"}]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:59.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270005#claim-300035","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270005#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:51.000Z"},{"id":"ptd-270004-300028","value":"wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"Source organism claim","claim_type":"organism","extracted_value":"trout","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'trout' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should be instructed to explicitly include the 'Verdict' field as provided in the optimized prompt, in addition to the 'Rationale'. For example, if the prompt provides 'Verdict: Insufficient Evidence', the model's output should start with 'Verdict: Insufficient Evidence' followed by the rationale.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300028","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300033","value":"These peptones were included (in the range of 2.6-11 g/L of soluble protein) as main source of organic nitrogen (protein substrates) in low-cost media for the culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria.","label":"Source organism claim","claim_type":"organism","extracted_value":"marine probiotic bacteria (MPB)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'marine probiotic bacteria (MPB)' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:47.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300033","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300026","value":"wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"Source organism claim","claim_type":"organism","extracted_value":"turbot","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'turbot' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300026","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300025","value":"peptones are produced from the Alcalase hydrolysis (0.1-0.2% v/w, 56-64 °C, pH 8.27-8.98, 3 h) and thermal processing (105 °C, 60 min) of wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"peptones produced from Alcalase hydrolysis and thermal processing of turbot, salmon, trout, seabream and seabass wastes","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'peptones produced from Alcalase hydrolysis and thermal processing of turbot, salmon, trout, seabream and seabass wastes' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300025","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300022","value":"Alcalase hydrolysis (0.1-0.2% v/w, 56-64 °C, pH 8.27-8.98, 3 h)","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Alcalase hydrolysis","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Alcalase hydrolysis' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should include the 'Verdict' field as provided in the optimized prompt. For example, it should output: 'Verdict: Insufficient Evidence. Rationale: Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Alcalase hydrolysis' not found in PubChem; [adapter] Manual review recommended']","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300022","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300023","value":"Alcalase hydrolysis (0.1-0.2% v/w, 56-64 °C, pH 8.27-8.98, 3 h)","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Alcalase","verdict":"Supported","verdict_rationale":"Source: CID:3086051 (confidence 85%). [Audit note: The model needs to output the 'Verdict' as well as the 'Rationale'. The prompt should explicitly state the required output format, e.g., 'Verdict: [Verdict] Rationale: [Rationale]' to ensure all components are included.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300023","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-supported"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300032","value":"These peptones were included (in the range of 2.6-11 g/L of soluble protein) as main source of organic nitrogen (protein substrates) in low-cost media for the culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria.","label":"Source organism claim","claim_type":"organism","extracted_value":"lactic acid bacteria (LAB)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'lactic acid bacteria (LAB)' not found in PubChem; [adapter] Manual review recommended [Audit note: The model must include the verdict in its output, consistent with the provided rationale. For example, it should output 'Verdict: Insufficient Evidence' followed by the rationale.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:48.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300032","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300024","value":"thermal processing (105 °C, 60 min)","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"thermal processing","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'thermal processing' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should include the 'Verdict' as specified in the optimized prompt, in addition to the 'Rationale'. For example, it should output: \"Verdict: Insufficient Evidence. Rationale: Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'thermal processing' not found in PubChem; [adapter] Manual review recommended\"]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300024","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300027","value":"wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"Source organism claim","claim_type":"organism","extracted_value":"salmon","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'salmon' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300027","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300030","value":"wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"Source organism claim","claim_type":"organism","extracted_value":"seabass","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'seabass' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:48.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300030","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300034","value":"These peptones were included (in the range of 2.6-11 g/L of soluble protein) as main source of organic nitrogen (protein substrates) in low-cost media for the culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria.","label":"Source organism claim","claim_type":"organism","extracted_value":"Gram+ bacteria","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Gram+ bacteria' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:48.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300034","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300031","value":"These peptones were included (in the range of 2.6-11 g/L of soluble protein) as main source of organic nitrogen (protein substrates) in low-cost media for the culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"peptones used as main source of organic nitrogen (protein substrates) for culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'peptones used as main source of organic nitrogen (protein substrates) for culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should explicitly state the verdict, for example: 'Verdict: Insufficient Evidence. Rationale: Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'peptones used as main source of organic nitrogen (protein substrates) for culture of lactic acid bacteria (LAB), marine probiotic bacteria (MPB) and ubiquitous Gram+ bacteria' not found in PubChem; [adapter] Manual review recommended']","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:48.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300031","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270004-300029","value":"wastes generated from the industrial processing of turbot, salmon, trout, seabream and seabass.","label":"Source organism claim","claim_type":"organism","extracted_value":"seabream","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'seabream' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#claim-300029","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270004#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:33.000Z"},{"id":"ptd-270003-300013","value":"Trimethylamine (TMA) is a known contributor to malodor in fish.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Trimethylamine (TMA) is a known contributor to malodor in fish","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Trimethylamine (TMA) is a known contributor to malodor in fish' not found in PubChem; [adapter] Manual review recommended [Audit note: Ensure the model output includes both the 'Verdict:' line and the 'Rationale:' line as specified in the optimized prompt. The model should output: 'Verdict: Insufficient Evidence Rationale: Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Trimethylamine (TMA) is a known contributor to malodor in fish' not found in PubChem; [adapter] Manual review recommended']","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:13.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300013","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300021","value":"We suggest an enzymatic solution for converting trimethylamine to the odorless trimethylamine N-oxide as a novel strategy to improve the smell quality of marine protein hydrolysates.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Enzymatic conversion of trimethylamine to trimethylamine N-oxide","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Enzymatic conversion of trimethylamine to trimethylamine N-oxide' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:19.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300021","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300015","value":"We identified a diverse set of bacterial Tmms using a sequence similarity network.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"sequence similarity network","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'sequence similarity network' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:09.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300015","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300019","value":"The enzymes were all active from pH 6.0 to 8.5, with functional stability being lowest around the optimal pH.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Enzymes active from pH 6.0 to 8.5","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Enzymes active from pH 6.0 to 8.5' not found in PubChem; [adapter] Manual review recommended [Audit note: The model must include the 'Verdict' field in its output, consistent with the optimized prompt's structure. For example, 'Verdict: Insufficient Evidence'.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:12.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300019","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300017","value":"From the 45 bacterial Tmm candidates investigated, eight enzymes from four different taxa were selected for their high activity toward TMA.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Eight bacterial Tmm enzymes from four different taxa showed high activity toward TMA","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Eight bacterial Tmm enzymes from four different taxa showed high activity toward TMA' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:11.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300017","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300020","value":"All three Tmms, given sufficient NADPH cofactor, were found to generate TMAO in the TMA-rich salmon protein hydrolysate.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Three Tmms generate TMAO in TMA-rich salmon protein hydrolysate with NADPH cofactor","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Three Tmms generate TMAO in TMA-rich salmon protein hydrolysate with NADPH cofactor' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:11.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300020","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300014","value":"we propose a novel enzymatic strategy to convert TMA into the odorless trimethylamine N-oxide (TMAO) using TMA monooxygenases (Tmms).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"TMA monooxygenases (Tmms) convert TMA into trimethylamine N-oxide (TMAO)","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'TMA monooxygenases (Tmms) convert TMA into trimethylamine N-oxide (TMAO)' not found in PubChem; [adapter] Manual review recommended [Audit note: Ensure the model output includes both the 'Verdict' and 'Rationale' fields as demonstrated in the optimized prompt. The verdict should be one of the specified categories (e.g., 'Insufficient Evidence').]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:10.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300014","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300016","value":"Purified, recombinant enzymes were assessed for their biocatalytic capacity by monitoring NADPH consumption and TMAO generation.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"monitoring NADPH consumption and TMAO generation","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'monitoring NADPH consumption and TMAO generation' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:10.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300016","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270003-300018","value":"The three most active enzymes were shown to vary in temperature optimum, with the highest being 45°C.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"The highest temperature optimum for the three most active enzymes was 45°C","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'The highest temperature optimum for the three most active enzymes was 45°C' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:51:10.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#claim-300018","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270003#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:51:03.000Z"},{"id":"ptd-270002-300012","value":"Secondary product: salmon collagen for nutraceutical, cosmetic, and wound care applications","label":"Protein name claim","claim_type":"protein_name","extracted_value":"salmon collagen","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'salmon collagen' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:19.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300012","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270002-300011","value":"Global Atlantic salmon production of approximately 3.0 million tonnes generates 1.5 million tonnes of byproducts annually, containing an estimated 180,000–225,000 tonnes of recoverable protein","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"180,000–225,000 tonnes of recoverable protein from Atlantic salmon byproducts","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound '180,000–225,000 tonnes of recoverable protein from Atlantic salmon byproducts' not found in PubChem; [adapter] Manual review recommended [Audit note: The prompt should be rephrased to clearly indicate what 'model answer' is expected in this context. If the model is supposed to *generate* the rationale for the 'Insufficient Evidence' verdict, then the provided 'Model answer' is correct. However, if the model is supposed to *critique* the provided 'Rationale' from the optimized prompt, then the current 'Model answer' is incorrect. The current setup makes it seem like the 'Model answer' *is* the rationale from the prompt, which means the model is not performing any task, but rather just echoing the input. Clarify if the 'Model answer' is an example of what *should* be generated or if it's an output to be critiqued. If it's an output to be critiqued, then the model should provide a critique of the given rationale against the validation criteria.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:19.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300011","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270002-300009","value":"salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone' not found in PubChem; [adapter] Manual review recommended [Audit note: The model needs to include the 'Verdict' field in its output, consistent with the optimized prompt's example. The prompt should explicitly state that both 'Verdict' and 'Rationale' are required fields for the output.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:15.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300009","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270002-300007","value":"fish-derived peptones match or exceed the performance of bovine and vegetable alternatives","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"fish-derived peptones match or exceed the performance of bovine and vegetable alternatives","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'fish-derived peptones match or exceed the performance of bovine and vegetable alternatives' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:18.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300007","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270002-300008","value":"fish are explicitly exempt from transmissible spongiform encephalopathy (TSE) regulations under EU Regulation 722/2012 and FDA guidance because they are not TSE-susceptible species","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"fish are not TSE-susceptible species","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'fish are not TSE-susceptible species' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:18.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300008","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270002-300010","value":"containing superior methionine and leucine profiles plus omega-3 fatty acids absent from all terrestrial sources","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"superior methionine and leucine profiles plus omega-3 fatty acids","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'superior methionine and leucine profiles plus omega-3 fatty acids' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:27:16.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#claim-300010","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270002#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:27:09.000Z"},{"id":"ptd-270001-300004","value":"containing superior methionine and leucine profiles plus omega-3 fatty acids absent from all terrestrial sources","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"salmon head peptone containing superior methionine and leucine profiles plus omega-3 fatty acids","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'salmon head peptone containing superior methionine and leucine profiles plus omega-3 fatty acids' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should be instructed to explicitly output both the 'Verdict' and 'Rationale' sections as provided in the optimized prompt. For example, 'Verdict: Insufficient Evidence. Rationale: Source completeness gate failed...'.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:26:55.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300004","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-270001-300005","value":"Global Atlantic salmon production of approximately 3.0 million tonnes generates 1.5 million tonnes of byproducts annually, containing an estimated 180,000–225,000 tonnes of recoverable protein","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Atlantic salmon byproducts contain 180,000–225,000 tonnes of recoverable protein annually","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Atlantic salmon byproducts contain 180,000–225,000 tonnes of recoverable protein annually' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should be instructed to output both the 'Verdict' and 'Rationale' as separate fields, consistent with the optimized prompt's structure. For example, 'Verdict: Insufficient Evidence. Rationale: [model output]'. The current prompt provides the verdict, so the model should be instructed to include it in its output.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:26:53.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300005","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-270001-300003","value":"salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'salmon head peptone performs equivalently to commercial animal peptone in recombinant protein production (p > 0.05) and actually outperforms vegetable peptone' not found in PubChem; [adapter] Manual review recommended [Audit note: The model should be instructed to always provide the 'Verdict' field, as it is a core component of the expected output structure and crucial for satisfying the deep intent of evaluating a claim. The prompt should explicitly state that the output format should match the 'Verdict: [value]\\nRationale: [value]' structure, or ideally, a JSON structure including both fields.]","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:26:54.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300003","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-270001-300002","value":"Fish are explicitly exempt from transmissible spongiform encephalopathy (TSE) regulations under EU Regulation 722/2012 and FDA guidance because they are not TSE-susceptible species","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Fish are not TSE-susceptible species","verdict":"Supported","verdict_rationale":"EU Commission Regulation No 722/2012 Article 1(2) explicitly limits TSE requirements to bovine, ovine, caprine species, deer, elk, mink and cats. Fish including Atlantic salmon are not listed and are therefore explicitly exempt. Source: CELEX:32012R0722.","manually_reviewed":true,"evidence_checked_at":"2026-06-10T09:26:54.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300002","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-supported"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-270001-300001","value":"fish-derived peptones match or exceed the performance of bovine and vegetable alternatives","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"fish-derived peptones match or exceed the performance of bovine and vegetable alternatives","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'fish-derived peptones match or exceed the performance of bovine and vegetable alternatives' not found in PubChem; [adapter] Manual review recommended","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:26:53.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300001","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-270001-300006","value":"Sequential extraction recovers collagen from skin and bones first, then hydrolyzes residual protein into peptone","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Sequential extraction recovers collagen from skin and bones first, then hydrolyzes residual protein into peptone","verdict":"Insufficient Evidence","verdict_rationale":"Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Sequential extraction recovers collagen from skin and bones first, then hydrolyzes residual protein into peptone' not found in PubChem; [adapter] Manual review recommended [Audit note: The model must include the 'Verdict' field as specified in the optimized prompt. For example, it should output: 'Verdict: Insufficient EvidenceRationale: Source completeness gate failed: Primary source not found; Required field absent in source record; Source data may be stale; [adapter] Compound 'Sequential extraction recovers collagen from skin and bones first, then hydrolyzes residual protein into peptone' not found in PubChem; [adapter] Manual review recommended']","manually_reviewed":false,"evidence_checked_at":"2026-06-10T09:26:54.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#claim-300006","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/270001#verdict-insufficient-evidence"],"date_observed":"2026-06-10T09:26:47.000Z"},{"id":"ptd-60110-121564","value":"The structure used in this study was PDB ID: 3V2A, an X-ray crystallographic structure of the VEGFR2 extracellular ligand-binding region in complex with VEGF-A, determined at a resolution of 3.20 A.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121564","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121544","value":"The receptor structure was obtained from Protein Data Bank and the structure was checked using ERRAT (81.176) along with Ramachandran analysis which resulted in 79.8% favored residues.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"79.8% favored residues","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121544","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121548","value":"A curated library of twenty-five cyclotides was modelled using AlphaFold, of which cycloviolacin O13 showed the best interaction with VEGFR2 as judged by a HADDOCK score of -84.7 and a ligand root-mean-squared deviation of 0.8 nm.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"HADDOCK score of -84.7","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121548","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121545","value":"A curated library of twenty-five cyclotides was modelled using AlphaFold, of which cycloviolacin O13 showed the best interaction with VEGFR2 as judged by a HADDOCK score of -84.7 and a ligand root-mean-squared deviation of 0.8 nm.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"AlphaFold","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121545","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121561","value":"The VEGFR2 receptor structure was retrieved from the Protein Data Bank (PDB).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121561","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121584","value":"Only 0.4% of residues were observed in generously allowed regions and 0.4% in disallowed regions.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"0.4% of residues in disallowed regions","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121584","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121565","value":"The structure used in this study was PDB ID: 3V2A, an X-ray crystallographic structure of the VEGFR2 extracellular ligand-binding region in complex with VEGF-A, determined at a resolution of 3.20 A.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"VEGF-A","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121565","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121568","value":"Stereochemical quality analysis was performed on the cleaned protein by applying PROCHECK (https://services.mbi.ucla.edu/PROCHECK/), Ramachandran analysis and ERRAT (https://services.mbi.ucla.edu/ERRAT/), non-bonded interaction analysis23,24.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"PROCHECK","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121568","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121576","value":"The VEGFR2 structure was computationally preprocessed (VEGF-A removal, missing residue correction, protonation, and energy minimization) prior to docking.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"VEGF-A","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121576","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121575","value":"The VEGFR2 structure was computationally preprocessed (VEGF-A removal, missing residue correction, protonation, and energy minimization) prior to docking.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121575","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121582","value":"When considered together, 99.2% of residues occupy sterically permissible conformational space, which exceeds the commonly accepted threshold of > 90–95% for reliable protein structural models.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"99.2% of residues occupy sterically permissible conformational space","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121582","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121581","value":"A total of 79.8% of residues were located in the most favored regions, while 19.4% were present in additionally allowed regions.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"79.8% of residues in most favored regions","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121581","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121588","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121588","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121587","value":"The results of a comparative MD analysis based on the geometry of the VEGFR2-cycloviolacinO13 complex, apo VEGFR2 as well as the free peptide showed that cycloviolacinO13 pro-stabilizes the receptor and is computationally prioritized for its biological activity relevant to wound-site angiogenesis.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacinO13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121587","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121591","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"MD simulations over 500 ns","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121591","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121594","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121594","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121562","value":"The structure used in this study was PDB ID: 3V2A, an X-ray crystallographic structure of the VEGFR2 extracellular ligand-binding region in complex with VEGF-A, determined at a resolution of 3.20 A.","label":"PDB structure identifier: 3V2A","claim_type":"pdb_id","extracted_value":"3V2A","verdict":"Supported","verdict_rationale":"PDB ID 3V2A exists in RCSB PDB: \"VEGFR-2/VEGF-A COMPLEX STRUCTURE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"3V2A","url":"https://www.rcsb.org/structure/3V2A","description":"PDB entry for 3V2A"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121562","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-supported"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121558","value":"Immuno-informatics evaluations found that cycloviolacin O13 was non-antigenic, non-allergenic and nontoxic, with immune simulations showing no adverse B- or T-cell response.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121558","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121557","value":"Dynamic cross-corr and principal component analyses supported the coordinated motions and a stable conformational response to peptide binding, and normal mode analysis suggested that low deformation and high mechanical resilience occur.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"normal mode analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121557","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121556","value":"Dynamic cross-corr and principal component analyses supported the coordinated motions and a stable conformational response to peptide binding, and normal mode analysis suggested that low deformation and high mechanical resilience occur.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"principal component analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121556","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121547","value":"A curated library of twenty-five cyclotides was modelled using AlphaFold, of which cycloviolacin O13 showed the best interaction with VEGFR2 as judged by a HADDOCK score of -84.7 and a ligand root-mean-squared deviation of 0.8 nm.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121547","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121570","value":"Stereochemical quality analysis was performed on the cleaned protein by applying PROCHECK (https://services.mbi.ucla.edu/PROCHECK/), Ramachandran analysis and ERRAT (https://services.mbi.ucla.edu/ERRAT/), non-bonded interaction analysis23,24.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"ERRAT","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121570","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121569","value":"Stereochemical quality analysis was performed on the cleaned protein by applying PROCHECK (https://services.mbi.ucla.edu/PROCHECK/), Ramachandran analysis and ERRAT (https://services.mbi.ucla.edu/ERRAT/), non-bonded interaction analysis23,24.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Ramachandran analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121569","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121555","value":"Dynamic cross-corr and principal component analyses supported the coordinated motions and a stable conformational response to peptide binding, and normal mode analysis suggested that low deformation and high mechanical resilience occur.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Dynamic cross-corr analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121555","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121552","value":"Molecular dynamics simulations over a period of 500 nanoseconds (ns) verified the stability of the VEGFR2-O13 complex in which root mean square deviation (RMSD) values were stabilized at 0.25–0.45 nm, compact radius of gyration and a permanent hydrogen bond network involving 200 to 260 hydrogen bonds.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121552","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121551","value":"Molecular dynamics simulations over a period of 500 nanoseconds (ns) verified the stability of the VEGFR2-O13 complex in which root mean square deviation (RMSD) values were stabilized at 0.25–0.45 nm, compact radius of gyration and a permanent hydrogen bond network involving 200 to 260 hydrogen bonds.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121551","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121554","value":"Molecular dynamics simulations over a period of 500 nanoseconds (ns) verified the stability of the VEGFR2-O13 complex in which root mean square deviation (RMSD) values were stabilized at 0.25–0.45 nm, compact radius of gyration and a permanent hydrogen bond network involving 200 to 260 hydrogen bonds.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"200 to 260 hydrogen bonds","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121554","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121549","value":"A curated library of twenty-five cyclotides was modelled using AlphaFold, of which cycloviolacin O13 showed the best interaction with VEGFR2 as judged by a HADDOCK score of -84.7 and a ligand root-mean-squared deviation of 0.8 nm.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"ligand root-mean-squared deviation of 0.8 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121549","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121543","value":"The receptor structure was obtained from Protein Data Bank and the structure was checked using ERRAT (81.176) along with Ramachandran analysis which resulted in 79.8% favored residues.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Ramachandran analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121543","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121542","value":"The receptor structure was obtained from Protein Data Bank and the structure was checked using ERRAT (81.176) along with Ramachandran analysis which resulted in 79.8% favored residues.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"ERRAT score of 81.176","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121542","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121595","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"200–260 H-bonds","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121595","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121540","value":"cycloviolacin O13, a cyclotide of plant origin, as a potential modulator of human VEGFR2.","label":"Source organism claim","claim_type":"organism","extracted_value":"human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121540","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121539","value":"cycloviolacin O13, a cyclotide of plant origin, as a potential modulator of human VEGFR2.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121539","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121583","value":"Only 0.4% of residues were observed in generously allowed regions and 0.4% in disallowed regions.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"0.4% of residues in generously allowed regions","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121583","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121537","value":"vascular endothelial growth factor receptor 2 (VEGFR2).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121537","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121546","value":"A curated library of twenty-five cyclotides was modelled using AlphaFold, of which cycloviolacin O13 showed the best interaction with VEGFR2 as judged by a HADDOCK score of -84.7 and a ligand root-mean-squared deviation of 0.8 nm.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121546","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121598","value":"In direct contrast, apo-VEGFR2 showed larger drift and expansion (RMSD 0.30–0.55 nm, excursions to 0.60 nm; Rg rising toward 1.82–1.86 nm; 150–200 H-bonds), supporting a ligand-associated stabilization of the predicted pocket.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"Rg rising toward 1.82–1.86 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121598","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121538","value":"cycloviolacin O13, a cyclotide of plant origin, as a potential modulator of human VEGFR2.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121538","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121599","value":"In direct contrast, apo-VEGFR2 showed larger drift and expansion (RMSD 0.30–0.55 nm, excursions to 0.60 nm; Rg rising toward 1.82–1.86 nm; 150–200 H-bonds), supporting a ligand-associated stabilization of the predicted pocket.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"150–200 H-bonds","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121599","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121600","value":"It is also important to acknowledge that cycloviolacins, including cycloviolacin O13, have been reported in the literature as membrane-active peptides with antimicrobial and cytotoxic properties, particularly at higher concentrations50–52.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121600","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121597","value":"In direct contrast, apo-VEGFR2 showed larger drift and expansion (RMSD 0.30–0.55 nm, excursions to 0.60 nm; Rg rising toward 1.82–1.86 nm; 150–200 H-bonds), supporting a ligand-associated stabilization of the predicted pocket.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"RMSD 0.30–0.55 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121597","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121593","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"ligand RMSD 0.20–0.30 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121593","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121592","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"complex RMSD stabilized at 0.25–0.45 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121592","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121590","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"ligand RMSD 0.8 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121590","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121589","value":"In our computational pipeline, cycloviolacin O13 emerged as the lead cyclotide because it combined the strongest docking performance (HADDOCK score − 84.7, ligand RMSD 0.8 nm) with long-timescale complex persistence during 500 ns MD: complex RMSD stabilized at 0.25–0.45 nm, the binding pose remained tight (ligand RMSD 0.20–0.30 nm), the complex compacted (Rg drifting from 1.83 to 1.88 nm early to 1.72–1.76 nm after ~ 150 ns), and hydrogen-bonding remained sustained (200–260 H-bonds).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"HADDOCK score of -84.7","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121589","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121586","value":"The results of a comparative MD analysis based on the geometry of the VEGFR2-cycloviolacinO13 complex, apo VEGFR2 as well as the free peptide showed that cycloviolacinO13 pro-stabilizes the receptor and is computationally prioritized for its biological activity relevant to wound-site angiogenesis.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121586","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121585","value":"The results of a comparative MD analysis based on the geometry of the VEGFR2-cycloviolacinO13 complex, apo VEGFR2 as well as the free peptide showed that cycloviolacinO13 pro-stabilizes the receptor and is computationally prioritized for its biological activity relevant to wound-site angiogenesis.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"comparative MD analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121585","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121596","value":"In direct contrast, apo-VEGFR2 showed larger drift and expansion (RMSD 0.30–0.55 nm, excursions to 0.60 nm; Rg rising toward 1.82–1.86 nm; 150–200 H-bonds), supporting a ligand-associated stabilization of the predicted pocket.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121596","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121580","value":"Ramachandran plot analysis further supported the stereochemical quality of the VEGFR2 model.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121580","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121579","value":"Ramachandran plot analysis further supported the stereochemical quality of the VEGFR2 model.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Ramachandran plot analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121579","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121541","value":"The receptor structure was obtained from Protein Data Bank and the structure was checked using ERRAT (81.176) along with Ramachandran analysis which resulted in 79.8% favored residues.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"ERRAT","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121541","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121577","value":"The ERRAT analysis generated a quality score of 81.176, as presented in Fig. 1(b), which is deemed to be very okay in regards to such a large and complicated receptor.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"ERRAT analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121577","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121550","value":"Molecular dynamics simulations over a period of 500 nanoseconds (ns) verified the stability of the VEGFR2-O13 complex in which root mean square deviation (RMSD) values were stabilized at 0.25–0.45 nm, compact radius of gyration and a permanent hydrogen bond network involving 200 to 260 hydrogen bonds.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Molecular dynamics simulations","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121550","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121553","value":"Molecular dynamics simulations over a period of 500 nanoseconds (ns) verified the stability of the VEGFR2-O13 complex in which root mean square deviation (RMSD) values were stabilized at 0.25–0.45 nm, compact radius of gyration and a permanent hydrogen bond network involving 200 to 260 hydrogen bonds.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"RMSD values were stabilized at 0.25–0.45 nm","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121553","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121560","value":"These results identify cycloviolacin O13 as a structurally stable and immunologically low-risk lead that can form a persistent, dynamically stable complex with VEGFR2 at a predicted binding pocket.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121560","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121559","value":"These results identify cycloviolacin O13 as a structurally stable and immunologically low-risk lead that can form a persistent, dynamically stable complex with VEGFR2 at a predicted binding pocket.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"cycloviolacin O13","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121559","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121563","value":"The structure used in this study was PDB ID: 3V2A, an X-ray crystallographic structure of the VEGFR2 extracellular ligand-binding region in complex with VEGF-A, determined at a resolution of 3.20 A.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"X-ray crystallography","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121563","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121578","value":"The ERRAT analysis generated a quality score of 81.176, as presented in Fig. 1(b), which is deemed to be very okay in regards to such a large and complicated receptor.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"ERRAT quality score of 81.176","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121578","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121566","value":"The structure used in this study was PDB ID: 3V2A, an X-ray crystallographic structure of the VEGFR2 extracellular ligand-binding region in complex with VEGF-A, determined at a resolution of 3.20 A.","label":"Crystal structure resolution","claim_type":"resolution","extracted_value":"3.20","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121566","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121567","value":"The VEGF-A ligand was removed prior to docking to allow evaluation of cyclotide binding within the receptor structure.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"VEGF-A","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121567","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121572","value":"To further validate the structural quality of the VEGFR2 receptor, ProSA-web analysis was performed to evaluate the overall model quality and calculate the Z-score relative to experimentally determined structures.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"ProSA-web analysis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121572","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121571","value":"To further validate the structural quality of the VEGFR2 receptor, ProSA-web analysis was performed to evaluate the overall model quality and calculate the Z-score relative to experimentally determined structures.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121571","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121573","value":"The rationalization and expression of the human VEGFR2 receptor resulted in a high-quality three-dimensional model that is suitable in all further computational studies.","label":"Source organism claim","claim_type":"organism","extracted_value":"human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121573","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60110-121574","value":"The rationalization and expression of the human VEGFR2 receptor resulted in a high-quality three-dimensional model that is suitable in all further computational studies.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"VEGFR2","verdict":"Ambiguous","verdict_rationale":"Protein name \"VEGFR2\" matches 3 PDB entries (e.g. 7YPL, 7LSB, 7LSI). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#claim-121574","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60110#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:36.000Z"},{"id":"ptd-60131-121528","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"adiponectin","verdict":"Ambiguous","verdict_rationale":"Protein name \"adiponectin\" matches 3 PDB entries (e.g. 5LX9, 5LXA, 6KS1). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121528","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121503","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"calcitonin-related","verdict":"Ambiguous","verdict_rationale":"Protein name \"calcitonin-related\" matches 3 PDB entries (e.g. 7KNT, 7KNU, 3N7R). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121503","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121535","value":"Several studies have confirmed that the hypothalamic nucleus lateral tuberis pars ventralis (NLTv) is the putative homolog in teleosts based on the hypothalamic mRNA and protein expression for agrp1, npy, cart, and pomc in different fish species [22,24,30,43,45–57].","label":"Protein name claim","claim_type":"protein_name","extracted_value":"pomc","verdict":"Ambiguous","verdict_rationale":"Protein name \"pomc\" matches 1 PDB entries (e.g. 6TUB). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:46.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121535","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121533","value":"Several studies have confirmed that the hypothalamic nucleus lateral tuberis pars ventralis (NLTv) is the putative homolog in teleosts based on the hypothalamic mRNA and protein expression for agrp1, npy, cart, and pomc in different fish species [22,24,30,43,45–57].","label":"Protein name claim","claim_type":"protein_name","extracted_value":"npy","verdict":"Ambiguous","verdict_rationale":"Protein name \"npy\" matches 3 PDB entries (e.g. 5VKF, 1F8P, 1TZ4). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:45.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121533","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121524","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"serotonin","verdict":"Ambiguous","verdict_rationale":"Protein name \"serotonin\" matches 3 PDB entries (e.g. 8C20, 8C21, 6Y5A). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121524","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121529","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"tumor necrosis factor-alpha","verdict":"Ambiguous","verdict_rationale":"Protein name \"tumor necrosis factor-alpha\" matches 3 PDB entries (e.g. 5JXD, 6I50, 8GYN). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121529","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121526","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"thyroid hormones","verdict":"Ambiguous","verdict_rationale":"Protein name \"thyroid hormones\" matches 3 PDB entries (e.g. 7KJJ, 9DXP, 9DWY). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121526","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121522","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"orexin/hypocretin","verdict":"Ambiguous","verdict_rationale":"Protein name \"orexin/hypocretin\" matches 3 PDB entries (e.g. 6TOT, 6TP3, 1CQ0). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121522","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121517","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"glucokinase","verdict":"Ambiguous","verdict_rationale":"Protein name \"glucokinase\" matches 3 PDB entries (e.g. 1Q18, 1L2L, 1SZ2). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121517","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121513","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"pomc","verdict":"Ambiguous","verdict_rationale":"Protein name \"pomc\" matches 1 PDB entries (e.g. 6TUB). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121513","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121510","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"mTOR","verdict":"Ambiguous","verdict_rationale":"Protein name \"mTOR\" matches 3 PDB entries (e.g. 7PED, 7DKL, 1Z58). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121510","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121506","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"leptin receptors","verdict":"Ambiguous","verdict_rationale":"Protein name \"leptin receptors\" matches 3 PDB entries (e.g. 8DH9, 8DHA, 5LXG). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121506","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121521","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"urotensin","verdict":"Ambiguous","verdict_rationale":"Protein name \"urotensin\" matches 3 PDB entries (e.g. 6HVB, 6HVK, 6HVC). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121521","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121520","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"corticotropin-releasing hormone binding protein","verdict":"Ambiguous","verdict_rationale":"Protein name \"corticotropin-releasing hormone binding protein\" matches 3 PDB entries (e.g. 2RMG, 2L27, 2RMH). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121520","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121530","value":"Genes grouped in reward-based (hedonic) appetite control (e.g., endocannabinoid receptor and fatty acid amid hydrolase) displayed a","label":"Protein name claim","claim_type":"protein_name","extracted_value":"endocannabinoid receptor","verdict":"Ambiguous","verdict_rationale":"Protein name \"endocannabinoid receptor\" matches 3 PDB entries (e.g. 7Q2T, 4AZQ, 4AZN). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121530","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121518","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"cd36","verdict":"Ambiguous","verdict_rationale":"Protein name \"cd36\" matches 3 PDB entries (e.g. 8J3P, 5KTF, 5UPH). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121518","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121500","value":"Key components of appetite control, including melanocortin system and nutrient-sensing pathway, were present at 720 dd and elevated levels were observed at 920 dd.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"melanocortin system and nutrient-sensing pathway were present at 720 dd and elevated levels were observed at 920 dd","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121500","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121527","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"thyroid transporters","verdict":"Ambiguous","verdict_rationale":"Protein name \"thyroid transporters\" matches 3 PDB entries (e.g. 9GV5, 9GSZ, 9GF8). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:43.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121527","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121515","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"lxr","verdict":"Ambiguous","verdict_rationale":"Protein name \"lxr\" matches 3 PDB entries (e.g. 7U8B, 3KFC, 1PQ6). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121515","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121525","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"somatostatin","verdict":"Ambiguous","verdict_rationale":"Protein name \"somatostatin\" matches 3 PDB entries (e.g. 2MI1, 7XNA, 7XN9). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:42.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121525","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121534","value":"Several studies have confirmed that the hypothalamic nucleus lateral tuberis pars ventralis (NLTv) is the putative homolog in teleosts based on the hypothalamic mRNA and protein expression for agrp1, npy, cart, and pomc in different fish species [22,24,30,43,45–57].","label":"Protein name claim","claim_type":"protein_name","extracted_value":"cart","verdict":"Ambiguous","verdict_rationale":"Protein name \"cart\" matches 3 PDB entries (e.g. 1HY9, 8BGJ, 5MLR). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:46.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121534","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121507","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"ampk","verdict":"Ambiguous","verdict_rationale":"Protein name \"ampk\" matches 3 PDB entries (e.g. 4YEE, 4YEF, 3KH5). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121507","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121514","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"melanocortin receptors","verdict":"Ambiguous","verdict_rationale":"Protein name \"melanocortin receptors\" matches 3 PDB entries (e.g. 6W25, 6FCE, 8GY7). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121514","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121536","value":"All the fish were euthanized on-site with an overdose of metacaine (MS-222TM; MSD Animal Health, Netherlands) before being handled further.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"metacaine (MS-222TM)","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:46.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121536","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121523","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"galanin","verdict":"Ambiguous","verdict_rationale":"Protein name \"galanin\" matches 3 PDB entries (e.g. 7S3O, 7S3Q, 7S3R). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:41.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121523","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121519","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"corticotropin-releasing hormone/factor","verdict":"Ambiguous","verdict_rationale":"Protein name \"corticotropin-releasing hormone/factor\" matches 3 PDB entries (e.g. 2RMG, 2RME, 2RMD). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121519","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121512","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"cart","verdict":"Ambiguous","verdict_rationale":"Protein name \"cart\" matches 3 PDB entries (e.g. 1HY9, 8BGJ, 5MLR). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121512","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121511","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"npy","verdict":"Ambiguous","verdict_rationale":"Protein name \"npy\" matches 3 PDB entries (e.g. 5VKF, 1F8P, 1TZ4). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121511","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121509","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"creb","verdict":"Ambiguous","verdict_rationale":"Protein name \"creb\" matches 3 PDB entries (e.g. 4HTM, 2D82, 7D8H). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121509","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121508","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"akt","verdict":"Ambiguous","verdict_rationale":"Protein name \"akt\" matches 3 PDB entries (e.g. 8OZZ, 1Q0R, 9IS8). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121508","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121505","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"cholecystokinin receptors","verdict":"Ambiguous","verdict_rationale":"Protein name \"cholecystokinin receptors\" matches 3 PDB entries (e.g. 7F8U, 7F8Y, 7F8X). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121505","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121504","value":"Overall, genes that had a higher expression before first feeding (720 dd) compared to after feeding (920 dd) were grouped into calcitonin-related, neuromedins, second-order neuron receptors, adipose-related, hormone receptors (cholecystokinin receptors, leptin receptors), and signaling proteins of cellular metabolism and signaling (transcription factors ampk, akt, creb, mTOR) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"neuromedins","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"neuromedins\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121504","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121502","value":"As illustrated by the Venn diagram (Fig 1B), 357 appetite-related genes (Ensembl IDs) were present across all three normalized count files (720 dd, 920 dd, and merged 720 dd and 920 dd), while 90 were absent in any dataset (the complete list of appetite-related genes is provided in S2 Table).","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"357 appetite-related genes (Ensembl IDs) were present across all three normalized count files","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121502","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121501","value":"Before onset of first feeding, 16 appetite-related genes displayed a significant cyclic profile, where most had a periodicity of 20 h or 28 h.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"16 appetite-related genes displayed a significant cyclic profile","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121501","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121498","value":"In this study, whole-brain RNA sequencing was used to investigate transcriptomic changes over 48 h during endogenous yolk utilization (720 day degrees (dd)) and after the onset of exogenous feeding (920 dd) in Atlantic salmon (Salmo salar), focusing on appetite-related genes.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"whole-brain RNA sequencing","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121498","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121499","value":"In this study, whole-brain RNA sequencing was used to investigate transcriptomic changes over 48 h during endogenous yolk utilization (720 day degrees (dd)) and after the onset of exogenous feeding (920 dd) in Atlantic salmon (Salmo salar), focusing on appetite-related genes.","label":"Source organism claim","claim_type":"organism","extracted_value":"Atlantic salmon (Salmo salar)","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121499","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121516","value":"Genes that were higher expressed after the onset of exogenous feeding (920 dd) were grouped in appetite-regulating neuropeptides (npy, cart, pomc), melanocortin receptors, nutrient sensing (e.g., lxr, glut2, glucokinase, cd36), corticotropin-releasing hormone system (including corticotropin-releasing hormone/factor, corticotropin-releasing hormone binding protein, urotensin), second-order neurons (e.g., orexin/hypocretin, galanin, serotonin, somatostatin), thyroid hormone system (thyroid hormones, and thyroid transporters), and genes linked to metabolism (adiponectin, and tumor necrosis factor-alpha) (Fig 1C).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"glut2","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"glut2\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121516","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121532","value":"Several studies have confirmed that the hypothalamic nucleus lateral tuberis pars ventralis (NLTv) is the putative homolog in teleosts based on the hypothalamic mRNA and protein expression for agrp1, npy, cart, and pomc in different fish species [22,24,30,43,45–57].","label":"Protein name claim","claim_type":"protein_name","extracted_value":"agrp1","verdict":"Ambiguous","verdict_rationale":"Protein name \"agrp1\" matches 3 PDB entries (e.g. 6VYI, 8ESM, 8ETM). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:45.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121532","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60131-121531","value":"Genes grouped in reward-based (hedonic) appetite control (e.g., endocannabinoid receptor and fatty acid amid hydrolase) displayed a","label":"Protein name claim","claim_type":"protein_name","extracted_value":"fatty acid amid hydrolase","verdict":"Ambiguous","verdict_rationale":"Protein name \"fatty acid amid hydrolase\" matches 3 PDB entries (e.g. 2WWD, 1CT9, 1O0B). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:44.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#claim-121531","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60131#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:33.000Z"},{"id":"ptd-60109-121406","value":"The CDP-tyvelose 2-epimerase from Thermodesulfatator atlanticus (TaTyvE) catalyzes the C-2 epimerization of CDP-glucose to CDP-mannose.","label":"Source organism claim","claim_type":"organism","extracted_value":"Thermodesulfatator atlanticus","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:26.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121406","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121468","value":"Finally, the structures of LysoPS-bound GPR174-Gs and GPR174-Gi complexes were solved at resolutions of 2.0 Å and 3.4 Å, respectively","label":"Crystal structure resolution","claim_type":"resolution","extracted_value":"2.0 Å","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:30.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121468","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121434","value":"Data collection and refinement statistics are shown in Table S1, and the crystal structure was deposited in the PDB (PDB ID: 9RL0).","label":"Experimental determination method for 9RL0","claim_type":"experimental_method","extracted_value":"crystal structure","verdict":"Insufficient Evidence","verdict_rationale":"Cannot verify method: PDB ID 9RL0 not found.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121434","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121463","value":"We observed that LysoPS activation of GPR174 exhibits a strong coupling preference for Gs over Gi, with no detectable response through Gq and a weak response through G13","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gq","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gq\" matches 3 PDB entries (e.g. 9UST, 6YFP, 8T8T). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121463","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121464","value":"We observed that LysoPS activation of GPR174 exhibits a strong coupling preference for Gs over Gi, with no detectable response through Gq and a weak response through G13","label":"Protein name claim","claim_type":"protein_name","extracted_value":"G13","verdict":"Ambiguous","verdict_rationale":"Protein name \"G13\" matches 3 PDB entries (e.g. 3F7I, 2IUL, 7SF7). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121464","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121438","value":"These findings agree with native mass spectrometry data (Fig. S4), confirming a tetrameric assembly for TaTyvE.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TaTyvE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"TaTyvE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121438","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121439","value":"The TaTyvE crystal structure with bound NAD+ and CDP at 2.60 Å resolution.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TaTyvE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"TaTyvE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121439","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121441","value":"The TaTyvE crystal structure with bound NAD+ and CDP at 2.60 Å resolution.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"CDP","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121441","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121442","value":"The TaTyvE crystal structure with bound NAD+ and CDP at 2.60 Å resolution.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"crystal structure","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121442","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121445","value":"Comparison of TaTyvE to sugar nucleotide C4 epimerases (GalE (26), GlcNAcE (30, 31), and GlcAE (33)) reveals conserved features with regard to the interplay of conformational flexibility and structural preorganization in substrate binding and catalysis.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GalE","verdict":"Ambiguous","verdict_rationale":"Protein name \"GalE\" matches 3 PDB entries (e.g. 3EHE, 1NVY, 1MF5). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121445","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121446","value":"Comparison of TaTyvE to sugar nucleotide C4 epimerases (GalE (26), GlcNAcE (30, 31), and GlcAE (33)) reveals conserved features with regard to the interplay of conformational flexibility and structural preorganization in substrate binding and catalysis.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GlcNAcE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"GlcNAcE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121446","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121448","value":"The VAM segment, together with Gln205, contributes to stabilization of CDP–Man (Figs. 5C & S9).","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"CDP–Man","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121448","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121449","value":"Escherichia coli BL21(DE3) competent cells were prepared in-house.","label":"Source organism claim","claim_type":"organism","extracted_value":"Escherichia coli BL21(DE3)","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121449","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121415","value":"Molecular dynamics simulations and site-directed mutagenesis suggest that TaTyvE employs a dual catalytic architecture to control substrate specificity.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TaTyvE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"TaTyvE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:30.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121415","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121466","value":"Finally, the structures of LysoPS-bound GPR174-Gs and GPR174-Gi complexes were solved at resolutions of 2.0 Å and 3.4 Å, respectively","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GPR174","verdict":"Ambiguous","verdict_rationale":"Protein name \"GPR174\" matches 3 PDB entries (e.g. 7XV3, 8KH5, 20YC). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121466","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121489","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gβ1","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"Gβ1\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121489","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121490","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"HiBiT","verdict":"Ambiguous","verdict_rationale":"Protein name \"HiBiT\" matches 3 PDB entries (e.g. 9LUK, 7SNX, 8VHF). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121490","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121492","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gi subunits","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gi subunits\" matches 3 PDB entries (e.g. 1TBG, 6M8S, 6EG8). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121492","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121494","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Protein name claim","claim_type":"protein_name","extracted_value":"DNGαi","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"DNGαi\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121494","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121473","value":"By resolving a high-resolution cryo-EM structure of the GPR174-Gs complex, we captured long-residence water molecules bridging conserved motifs to the intracellular G protein-binding interface","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gs","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gs\" matches 3 PDB entries (e.g. 8PSH, 6OJY, 6YCS). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:31.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121473","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121497","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gγ2","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"Gγ2\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121497","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121414","value":"Molecular dynamics simulations and site-directed mutagenesis suggest that TaTyvE employs a dual catalytic architecture to control substrate specificity.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"site-directed mutagenesis","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121414","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121462","value":"We observed that LysoPS activation of GPR174 exhibits a strong coupling preference for Gs over Gi, with no detectable response through Gq and a weak response through G13","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gi","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gi\" matches 3 PDB entries (e.g. 8J5B, 1QS3, 2FR9). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:28.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121462","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121488","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"DNGαs","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"DNGαs\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121488","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121477","value":"An N-terminal hemagglutinin (HA) signal peptide and FLAG tag (DYKDDDDA) were introduced, followed by a thermostabilized BRIL to enhance receptor expression.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"hemagglutinin (HA) signal peptide","verdict":"Ambiguous","verdict_rationale":"Protein name \"hemagglutinin (HA) signal peptide\" matches 3 PDB entries (e.g. 6D8W, 8JRU, 1FYT). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:31.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121477","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121472","value":"By resolving a high-resolution cryo-EM structure of the GPR174-Gs complex, we captured long-residence water molecules bridging conserved motifs to the intracellular G protein-binding interface","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GPR174","verdict":"Ambiguous","verdict_rationale":"Protein name \"GPR174\" matches 3 PDB entries (e.g. 7XV3, 8KH5, 20YC). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:30.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121472","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121476","value":"wild-type (WT) human GPR174 (UniProt ID: Q9BXC1) was cloned into a modified pFastBac1 vector.","label":"General molecular biology claim","claim_type":"general_molecular","extracted_value":"UniProt ID: Q9BXC1","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:31.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121476","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121413","value":"Molecular dynamics simulations and site-directed mutagenesis suggest that TaTyvE employs a dual catalytic architecture to control substrate specificity.","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"Molecular dynamics simulations","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121413","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121460","value":"We observed that LysoPS activation of GPR174 exhibits a strong coupling preference for Gs over Gi, with no detectable response through Gq and a weak response through G13","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"LysoPS","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:28.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121460","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121483","value":"Human dominant-negative (DN) Gαs and DN Gαi1 were generated by site-directed mutagenesis as previously described to decrease the nucleotide-binding affinity and increase G protein stability","label":"Source organism claim","claim_type":"organism","extracted_value":"Human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121483","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121407","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"Crystal structure resolution for 9RL0","claim_type":"resolution","extracted_value":"2.60","verdict":"Insufficient Evidence","verdict_rationale":"Cannot verify resolution: PDB ID 9RL0 not found.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:27.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121407","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121475","value":"wild-type (WT) human GPR174 (UniProt ID: Q9BXC1) was cloned into a modified pFastBac1 vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GPR174","verdict":"Ambiguous","verdict_rationale":"Protein name \"GPR174\" matches 3 PDB entries (e.g. 7XV3, 8KH5, 20YC). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:31.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121475","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121486","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gs subunits","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gs subunits\" matches 3 PDB entries (e.g. 6EG8, 7XW5, 3SN6). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121486","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121471","value":"By resolving a high-resolution cryo-EM structure of the GPR174-Gs complex, we captured long-residence water molecules bridging conserved motifs to the intracellular G protein-binding interface","label":"Experimental determination method","claim_type":"experimental_method","extracted_value":"cryo-EM","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:30.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121471","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121405","value":"The CDP-tyvelose 2-epimerase from Thermodesulfatator atlanticus (TaTyvE) catalyzes the C-2 epimerization of CDP-glucose to CDP-mannose.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"CDP-tyvelose 2-epimerase","verdict":"Ambiguous","verdict_rationale":"Protein name \"CDP-tyvelose 2-epimerase\" matches 1 PDB entries (e.g. 1ORR). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:26.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121405","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121496","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Protein name claim","claim_type":"protein_name","extracted_value":"HiBiT","verdict":"Ambiguous","verdict_rationale":"Protein name \"HiBiT\" matches 3 PDB entries (e.g. 9LUK, 7SNX, 8VHF). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121496","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121493","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Source organism claim","claim_type":"organism","extracted_value":"human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:37.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121493","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121491","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gγ2","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"Gγ2\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:36.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121491","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121481","value":"The C terminus was fused to a LgBiT tag, a TEV protease cleavage site, and tandem maltose-binding protein (MBP) tags.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TEV protease","verdict":"Ambiguous","verdict_rationale":"Protein name \"TEV protease\" matches 3 PDB entries (e.g. 9N7Y, 1Q31, 9HXC). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:33.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121481","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121447","value":"Comparison of TaTyvE to sugar nucleotide C4 epimerases (GalE (26), GlcNAcE (30, 31), and GlcAE (33)) reveals conserved features with regard to the interplay of conformational flexibility and structural preorganization in substrate binding and catalysis.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"GlcAE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"GlcAE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:40.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121447","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121443","value":"The TaTyvE crystal structure with bound NAD+ and CDP at 2.60 Å resolution.","label":"Crystal structure resolution","claim_type":"resolution","extracted_value":"2.60","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121443","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121440","value":"The TaTyvE crystal structure with bound NAD+ and CDP at 2.60 Å resolution.","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"NAD+","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121440","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121465","value":"Finally, the structures of LysoPS-bound GPR174-Gs and GPR174-Gi complexes were solved at resolutions of 2.0 Å and 3.4 Å, respectively","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"LysoPS","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121465","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121409","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TaTyvE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"TaTyvE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:28.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121409","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121410","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"NAD+","verdict":"Insufficient Evidence","verdict_rationale":"Cannot verify ligand: PDB ID 9RL0 not found.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121410","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121461","value":"We observed that LysoPS activation of GPR174 exhibits a strong coupling preference for Gs over Gi, with no detectable response through Gq and a weak response through G13","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gs","verdict":"Ambiguous","verdict_rationale":"Protein name \"Gs\" matches 3 PDB entries (e.g. 8PSH, 6OJY, 6YCS). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:28.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121461","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121444","value":"Comparison of TaTyvE to sugar nucleotide C4 epimerases (GalE (26), GlcNAcE (30, 31), and GlcAE (33)) reveals conserved features with regard to the interplay of conformational flexibility and structural preorganization in substrate binding and catalysis.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"TaTyvE","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"TaTyvE\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:39.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121444","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121482","value":"The C terminus was fused to a LgBiT tag, a TEV protease cleavage site, and tandem maltose-binding protein (MBP) tags.","label":"Protein name claim","claim_type":"protein_name","extracted_value":"maltose-binding protein (MBP)","verdict":"Ambiguous","verdict_rationale":"Protein name \"maltose-binding protein (MBP)\" matches 3 PDB entries (e.g. 2R6G, 3PUZ, 3PV0). Specific PDB ID required for definitive verification.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:34.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121482","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-ambiguous"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121487","value":"All three Gs subunits, including human DNGαs, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned separately into the pFastBac vector.","label":"Source organism claim","claim_type":"organism","extracted_value":"human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:35.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121487","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121495","value":"Similarly, the three Gi subunits, including human DNGαi, WT Gβ1 fusion to HiBiT, and Gγ2, were cloned into the pFastBac vector separately","label":"Protein name claim","claim_type":"protein_name","extracted_value":"Gβ1","verdict":"Insufficient Evidence","verdict_rationale":"No PDB entries found matching protein name \"Gβ1\".","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:38.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121495","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121411","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"Ligand / small molecule claim","claim_type":"ligand","extracted_value":"CDP","verdict":"Insufficient Evidence","verdict_rationale":"Cannot verify ligand: PDB ID 9RL0 not found.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121411","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121412","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"PDB structure identifier: 9RL0","claim_type":"pdb_id","extracted_value":"9RL0","verdict":"Contradicted","verdict_rationale":"PDB ID 9RL0 does not exist in the RCSB Protein Data Bank.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:29.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121412","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-contradicted"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60109-121408","value":"Here, we report the 2.60-Å crystal structure of tetrameric TaTyvE with NAD+ bound in all subunits and CDP bound in one (Protein Data Bank code: 9RL0).","label":"Experimental determination method for 9RL0","claim_type":"experimental_method","extracted_value":"crystal structure","verdict":"Insufficient Evidence","verdict_rationale":"Cannot verify method: PDB ID 9RL0 not found.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:28.000Z","source_refs":[{"database":"RCSB Protein Data Bank","entry_id":"9RL0","url":"https://www.rcsb.org/structure/9RL0","description":"PDB entry for 9RL0"}],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#claim-121408","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60109#verdict-insufficient-evidence"],"date_observed":"2026-06-03T10:10:25.000Z"},{"id":"ptd-60067-121474","value":"wild-type (WT) human GPR174 (UniProt ID: Q9BXC1) was cloned into a modified pFastBac1 vector.","label":"Source organism claim","claim_type":"organism","extracted_value":"human","verdict":"Out of Scope","verdict_rationale":"This claim type cannot be automatically verified against the Protein Data Bank. Expert review recommended.","manually_reviewed":false,"evidence_checked_at":"2026-06-03T10:10:31.000Z","source_refs":[],"page_anchors":["https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#claim-121474","https://b2mbh3cn4a-w6dvs6ghja-uk.a.run.app/audit/60067#verdict-out-of-scope"],"date_observed":"2026-06-03T10:10:25.000Z"}]}